Beta actin kda western blot9/19/2023 Loading control rabbit anti beta-actin (antibodies-online, ABIN962807) diluted 1:500 in blocking buffer ON at 4☌. Block the membrane with blocking buffer (2% BSA/PBS/0.05%Tween-20) for 1h at RT.Transfer proteins onto PVDF membrane (Millipore, IPVH00010, lot K5AA6843U) with a Western blotting system for ON at 4☌ at 150mA.Denature 200µg total protein for 5min at 95☌ in 20µl Laemmli SDS sample buffer and subsequently separate them on a SDS-PAGE gel using Acrylamide/Bis Premixed (Bio-Rad, 61-0125, lot 260000477) for 2-3h at 100V.Determine total protein content of the lysates using Commassie Protein Assay Reagent (Thermo Scientific, 1856209, lot NL179252).Lyse cells in cold lysis buffer (10mM Tris pH7.4, 150mM NaCl, 0.5mM EDTA, 2% CHAPS).Transfect cells with pCDNA 3.1 neo (-) (Invitrogen) containing human MR1 cDNA (Genecopoeia) using Polyethylenimine (Polysciences, 23966) following the manufacturer´s instructions.It has not yet been tested in other species.Ĭross reactivity in other species has not yet been tested.Ī synthetic peptide DDDIAALVVDNGSG coupled - KLH THE TM beta Actin Antibody, mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig. Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) Immunofluorescence (fixed cells) (IF/ICC) Immunohistochemistry (Frozen Sections) (IHC (fro)) Immunofluorescence (Cultured Cells) (IF (cc)) Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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